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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VCAM-1 Monoclonal antibody, PBS Only

VCAM-1 Monoclonal Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B12F12

Cat no : 66294-1-PBS

Synonyms

CD106, INCAM 100, V CAM 1, VCAM 1, VCAM1, VCAM-1

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VCAM-1 Monoclonal antibody (66294-1-Ig)

    IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VCAM-1 Monoclonal antibody (66294-1-Ig)

    IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VCAM-1 Monoclonal antibody (66294-1-Ig)

    IHC staining of human lung cancer using 66294-1-Ig (same clone as 66294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VCAM-1 Monoclonal antibody (66294-1-Ig)

    IHC staining of human lung cancer using 66294-1-Ig (same clone as 66294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VCAM-1 Monoclonal antibody (66294-1-Ig)

    IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VCAM-1 Monoclonal antibody (66294-1-Ig)

    IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using VCAM-1 Monoclonal antibody (66294-1-Ig)

    IF Staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using VCAM-1 antibody (66294-1-Ig, Clone: 1B12F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using VCAM-1 Monoclonal antibody (66294-1-Ig)

    IF Staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using VCAM-1 antibody (66294-1-Ig, Clone: 1B12F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66294-1-PBS targets VCAM-1 in IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen VCAM-1 fusion protein Ag24697 相同性解析による交差性が予測される生物種
Full Name vascular cell adhesion molecule 1
Calculated molecular weight 739 aa, 81 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC017276
Gene symbol VCAM1
Gene ID (NCBI) 7412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).