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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse VCAM-1/CD106 Recombinant antibody, PBS Only (Capture/Detector)

VCAM-1/CD106 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240597D2

Cat no : 83719-3-PBS

Synonyms

CD106, VCAM-1, Vcam1, Vcam 1, 240597D2

Validation Data Gallery

Filter:
  • Sandwich ELISA standard curve of MP00701-4

    Sandwich ELISA standard curve of MP00701-4

    Sandwich ELISA standard curve of MP00701-4, Mouse VCAM-1/CD106 Recombinant Matched Antibody Pair - PBS only. 83719-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0639. 83719-3-PBS was HRP conjugated as the detection antibody. Range: 46.88-3000 pg/mL.

  • Sample test of MP00701-4

    Sample test of MP00701-4

    Urine of eight mice was measured. The VCAM-1 concentration of detected samples was determined to be 71.97 ng/mL with a range of 8.65-105.58 ng/mL.

  • Sample test of MP00701-4

    Sample test of MP00701-4

    Mouse lung, cut into 1-2 mm pieces, was cultured for 3 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and unstimulated or stimulated with 10 μg/mL ConA. The mean VCAM-1 concentration was determined to be 14.2 ng/mL in unstimulated mouse lung superantant, 20.9 ng/mL in ConA stimulated mouse lung superantant.

  • Sample test of MP00701-4

    Sample test of MP00701-4

    Serum of eight mice was measured. The VCAM-1 concentration of detected samples was determined to be 572.76 ng/mL with a range of 483.18-844.96 ng/mL.

  • Cytometric bead array standard curve of MP00701-2

    Cytometric bead array standard curve of MP00701-2

    Cytometric bead array standard curve of MP00701-2, MOUSE VCAM-1/CD106 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83719-3-PBS. Detection antibody: 83719-5-PBS. Standard: Eg0639. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00701-3

    Cytometric bead array standard curve of MP00701-3

    Cytometric bead array standard curve of MP00701-3, MOUSE VCAM-1/CD106 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83719-3-PBS. Detection antibody: 83719-2-PBS. Standard: Eg0639. Range: 0.313-40 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83719-3-PBS targets VCAM-1/CD106 as part of a matched antibody pair:

MP00701-2: 83719-3-PBS capture and 83719-5-PBS detection (validated in Cytometric bead array)

MP00701-3: 83719-3-PBS capture and 83719-2-PBS detection (validated in Cytometric bead array)

MP00701-4: 83719-1-PBS capture and 83719-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name vascular cell adhesion molecule 1
Calculated molecular weight81 kDa
GenBank accession numberCAA47989.1
Gene symbol VCAM-1
Gene ID (NCBI) 22329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.