• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VCAM-1/CD106 Recombinant antibody, PBS Only (Capture)

VCAM-1/CD106 Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241284B2

Cat no : 84249-4-PBS

Synonyms

VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HUVEC cells using VCAM-1/CD106 Recombinant antibody (84249-4-RR)

    WB analysis of HUVEC using 84249-4-RR (same clone as 84249-4-PBS)

    TNF alpha treated HUVEC cells were subjected to SDS PAGE followed by western blot with 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01145-3

    Sandwich ELISA standard curve of MP01145-3

    Sandwich ELISA standard curve of MP01145-3, Human VCAM-1/CD106 Recombinant Matched Antibody Pair - PBS only. 84249-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02345. 84249-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP01145-3

    Sample test of MP01145-3

    Serum of six individual healthy human donors was measured. The human VCAM-1/CD106 concentration of detected samples was determined to be 188.79 ng/mL with a range of 126.68 - 336.06 ng/mL.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VCAM-1/CD106 Recombinant antibody (84249-4-RR)

    IHC staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VCAM-1/CD106 Recombinant antibody (84249-4-RR)

    IHC staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01145-3

    Cytometric bead array standard curve of MP01145-3

    Cytometric bead array standard curve of MP01145-3, VCAM-1/CD106 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84249-4-PBS. Detection antibody: 84249-2-PBS. Standard: RP02345. Range: 0.625-40 ng/mL.

☆1/31（金）までお得に買える！ >> IHC・ELISA・IPキット最大30%OFFキャンペーン

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84249-4-PBS targets VCAM-1/CD106 as part of a matched antibody pair:

MP01145-3: 84249-4-PBS capture and 84249-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name vascular cell adhesion molecule 1
Calculated molecular weight81 kDa
Observed molecular weight100 kDa
GenBank accession numberNM_001078.4
Gene symbol VCAM-1
Gene ID (NCBI) 7412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).