Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human stomach cancer tissue, human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30599-1-AP targets VCAN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VCAN fusion protein Ag30151 相同性解析による交差性が予測される生物種 |
| Full Name | versican |
| Calculated molecular weight | 373 kDa |
| Observed molecular weight | 250-300 kDa, 550 kDa |
| GenBank accession number | NM_004385 |
| Gene Symbol | VCAN |
| Gene ID (NCBI) | 1462 |
| RRID | AB_3669736 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13611 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Versican, a component of the extracellular matrix and a member of the lectican family of proteoglycans, is the largest chondroitin sulfate proteoglycan and has an important role in the development of the heart, musculoskeletal system and central nervous system (CNS). The structure of versican is characterized by an approximately 550-kDa core protein consisting of an amino-terminal (G1) hyaluronan binding domain, a carboxy-terminal (G3) domain, and two central chondroitin sulfate glycosaminoglycan attachment domains, the α-GAG and β-GAG domains. Versican has four different isoforms that differ in the central GAG attachment domain: V0 (contains both α and β GAG domains), V1 (contains only the β domain), V2 (contains only the α domain) and V3 (devoid of both GAG domains)(PMID: 26385570).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VCAN antibody 30599-1-AP | Download protocol |
| IHC protocol for VCAN antibody 30599-1-AP | Download protocol |
| IF protocol for VCAN antibody 30599-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |