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KD/KO Validated

VCP Polyclonal antibody

Name: VCP Polyclonal antibody
Brand: Proteintech
SKU: 10736-1-AP

VCP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10736-1-AP

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

Validation Data Gallery

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AllWBIPIHC

WB analysis of U2OS using 10736-1-AP
U2OS (WT and VCP KD) lysates prepared with RIPA buffer, 10μg protein loaded. 10736-1-AP incubated at 1:15000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
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U2OS (WT and VCP KD) lysates prepared with RIPA buffer, 10μg protein loaded. 10736-1-AP incubated at 1:15000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
WB analysis using 10736-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 10736-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 10736-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 10736-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10736-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 10736-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10736-1-AP
IP result of anti-VCP (IP:10736-1-AP, 4ug; Detection:10736-1-AP 1:1000) with HeLa cells lysate 1505ug.
IP experiment of mouse brain using 10736-1-AP
IP result of anti-VCP (IP:10736-1-AP, 4ug; Detection:10736-1-AP 1:300) with mouse brain tissue lysate 2400 ug.
IHC staining of human gliomas using 10736-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10736-1-AP (VCP Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human gliomas using 10736-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10736-1-AP (VCP Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human gliomas using 10736-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human gliomas using 10736-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human gliomas using 10736-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 10736-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 10736-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 10736-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 10736-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 10736-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, rat lung tissue, mouse lung tissue, U2OS cells, C6 cells, mouse thymus tissue, mouse brain tissue, rat brain tissue, SH-SY5Y cells, NIH/3T3 cells
Positive IP detected in	HeLa cells, mouse brain tissue
Positive IHC detected in	human gliomas tissue, human colon cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 43 publications below
IHC	See 1 publications below
IF	See 5 publications below
CoIP	See 1 publications below

Product Information

10736-1-AP targets VCP in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VCP fusion protein Ag1002 相同性解析による交差性が予測される生物種
Full Name	valosin-containing protein
Calculated molecular weight	89 kDa
Observed molecular weight	90-100 kDa
GenBank accession number	BC007562
Gene symbol	VCP
Gene ID (NCBI)	7415
RRID	AB_2214635
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Protocols

Product Specific Protocols
WB protocol for VCP antibody 10736-1-AP	Download protocol
IHC protocol for VCP antibody 10736-1-AP	Download protocol
IP protocol for VCP antibody 10736-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival Authors - Pauline Lascaux View Article
mouse	WB	Cell Metab AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity. Authors - Hui Luo View Article
human	WB	J Clin Invest Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders Authors - Huilun Helen Wang View Article
human	WB	Sci Adv Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor. Authors - Yuan Wang View Article
human	WB	Nat Commun Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection. Authors - Rommel J Gestuveo KD Validated View Article
human	WB	Cell Rep hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility Authors - Xiaoli Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VCP Polyclonal antibody

VCP Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of U2OS using 10736-1-AP

WB analysis using 10736-1-AP

WB analysis of rat lung using 10736-1-AP

WB analysis of mouse lung using 10736-1-AP

WB analysis of mouse thymus using 10736-1-AP

WB analysis of human brain using 10736-1-AP

WB analysis using 10736-1-AP

IP experiment of HeLa using 10736-1-AP

IP experiment of mouse brain using 10736-1-AP

IHC staining of human gliomas using 10736-1-AP

IHC staining of human gliomas using 10736-1-AP

IHC staining of human gliomas using 10736-1-AP

IHC staining of human gliomas using 10736-1-AP

IHC staining of human gliomas using 10736-1-AP

IHC staining of human colon cancer using 10736-1-AP

IHC staining of human colon cancer using 10736-1-AP

IHC staining of human lung cancer using 10736-1-AP

IHC staining of human lung cancer using 10736-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders

Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.

Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection.

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility