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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VCP Recombinant antibody, PBS Only

VCP Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G23

Cat no : 82463-1-PBS

Synonyms

HEL-S-70, HEL-220, EC:3.6.4.6, 3G23, 15S Mg(2+)-ATPase p97 subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VCP Recombinant antibody (82463-1-RR)

    WB analysis using 82463-1-RR (same clone as 82463-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82463-1-RR (VCP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using VCP Recombinant antibody (82463-1-RR)

    IP experiment of HeLa using 82463-1-RR (same clone as 82463-1-PBS)

    IP result of anti-VCP (IP:82463-1-RR, 4ug; Detection:82463-1-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using VCP Recombinant antibody (82463-1-RR)

    IHC staining of rat small intestine using 82463-1-RR (same clone as 82463-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 82463-1-RR (VCP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82463-1-PBS

    Affinity and Kinetic Characterization of 82463-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82463-1-RR against Human VCP were performed. The affinity constant is 0.997 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82463-1-PBS targets VCP in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen VCP fusion protein Ag1002 相同性解析による交差性が予測される生物種
Full Name valosin-containing protein
Calculated molecular weight 89 kDa
Observed molecular weight89 kDa
GenBank accession numberBC007562
Gene symbol VCP
Gene ID (NCBI) 7415
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).