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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VCPIP1 Polyclonal antibody

VCPIP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, RIP, ELISA

Conjugate

Unconjugated

Cat no : 17802-1-AP

Synonyms

DUBA3, KIAA1850, VCIP135, VCPIP1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using VCPIP1 Polyclonal antibody (17802-1-AP)

    WB analysis of MCF-7 using 17802-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17802-1-AP (VCPIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using VCPIP1 Polyclonal antibody (17802-1-AP)

    IHC staining of human ovary tumor using 17802-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17802-1-AP (VCPIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VCPIP1 Polyclonal antibody (17802-1-AP)

    IHC staining of human breast cancer using 17802-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17802-1-AP (VCPIP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using VCPIP1 Polyclonal antibody (17802-1-AP)

    IHC staining of human ovary tumor using 17802-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17802-1-AP (VCPIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VCPIP1 Polyclonal antibody (17802-1-AP)

    IHC staining of human breast cancer using 17802-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17802-1-AP (VCPIP1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inMCF7 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17802-1-AP is VCPIP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VCPIP1 fusion protein Ag12035 相同性解析による交差性が予測される生物種
Full Name valosin containing protein (p97)/p47 complex interacting protein 1
Calculated molecular weight 1222 aa, 134 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC094799
Gene symbol VCPIP1
Gene ID (NCBI) 80124
RRIDAB_10699873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VCPIP1 antibody 17802-1-APDownload protocol
IHC protocol for VCPIP1 antibody 17802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Immunol Res

USP22 deubiquitinates CD274 to suppress anti-cancer immunity.

Authors - Xing Huang
View Article
humanRIP

Genes Dis

Downregulation of nc886 contributes to prostate cancer cell invasion and TGFβ1-induced EMT.

Authors - Ronghui Yang
View Article