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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VDAC1/Porin Monoclonal antibody (66345-1-Ig)

    WB analysis using 66345-1-Ig (same clone as 66345-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66345-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using VDAC1/Porin Monoclonal antibody (66345-1-Ig)

    IF Staining of mouse liver using 66345-1-Ig (same clone as 66345-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using VDAC1/Porin antibody (66345-1-Ig, Clone: 1E2C7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66345-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using VDAC1/Porin Monoclonal antibody (66345-1-Ig)

    FC experiment of HepG2 using 66345-1-Ig (same clone as 66345-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug VDAC1/Porin Monoclonal antibody (66345-1-Ig, Clone:1E2C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG3 isotype control Mouse McAb (66360-4-Ig, Clone: 1H4A5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66345-1-PBS in a different storage buffer formulation.

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VDAC1/Porin Monoclonal antibody, PBS Only

VDAC1/Porin Monoclonal Antibody for WB, IF-P, FC (Intra), Indirect ELISA
Cat No. 66345-1-PBS
Clone No.1E2C7

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, rat

Applications

WB, IF-P, FC (Intra), Indirect ELISA

Synonyms

Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66345-1-PBS targets VDAC1/Porin in WB, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name voltage-dependent anion channel 1
Calculated molecular weight 31 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberNM_003374
Gene Symbol Porin
Gene ID (NCBI) 7416
RRIDAB_2881725
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21796
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904).