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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VDAC1/Porin Recombinant antibody, PBS Only

VDAC1/Porin Recombinant Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F4

Cat no : 81538-1-PBS

Synonyms

Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VDAC1/Porin Recombinant antibody (81538-1-RR)

    WB analysis using 81538-1-RR (same clone as 81538-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81538-1-RR (VDAC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81538-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using VDAC1/Porin Recombinant antibody (81538-1-RR)

    IHC staining of mouse liver using 81538-1-RR (same clone as 81538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81538-1-RR (VDAC1/Porin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81538-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using VDAC1/Porin Recombinant antibody (81538-1-RR)

    IHC staining of mouse liver using 81538-1-RR (same clone as 81538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81538-1-RR (VDAC1/Porin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81538-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using VDAC1/Porin Recombinant antibody (81538-1-RR)

    IF Staining of human liver using 81538-1-RR (same clone as 81538-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using VDAC1/Porin antibody (81538-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81538-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using VDAC1/Porin Recombinant antibody (81538-1-RR)

    FC experiment of HepG2 using 81538-1-RR (same clone as 81538-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug VDAC1/Porin Recombinant antibody (81538-1-RR, Clone:2F4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81538-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81538-1-PBS targets VDAC1/Porin in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name voltage-dependent anion channel 1
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberNM_003374
Gene symbol Porin
Gene ID (NCBI) 7416
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.