Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, human heart tissue, mouse heart tissue, rat brain tissue, rat heart tissue |
| Positive IHC detected in | human prostate cancer tissue, human normal colon, human prostate tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 35 publications below |
| IHC | See 3 publications below |
| IF | See 7 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
11663-1-AP targets VDAC1/2/3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2266 Product name: Recombinant human VDAC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-294 aa of BC000165 Sequence: MATHGQTCARPMCIPPSYADLGKAARDIFNKGFGFGLVKLDVKTKSCSGVEFSTSGSSNTDTGKVTGTLETKYKWCEYGLTFTEKWNTDNTLGTEIAIEDQICQGLKLTFDTTFSPNTGKKSGKIKSSYKRECINLGCDVDFDFAGPAIHGSAVFGYEGWLAGYQMTFDSAKSKLTRNNFAVGYRTGDFQLHTNVNDGTEFGGSIYQKVCEDLDTSVNLAWTSGTNCTRFGIAAKYQLDPTASISAKVNNSSLIGVGYTQTLRPGVKLTLSALVDGKSINAGGHKVGLALELEA 相同性解析による交差性が予測される生物種 |
| Full Name | voltage-dependent anion channel 2 |
| Calculated molecular weight | 294 aa, 32 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC000165 |
| Gene Symbol | VDAC2 |
| Gene ID (NCBI) | 7417 |
| RRID | AB_2304144 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P45880 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and expecially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue.(PMID: 22020053)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VDAC1/2/3 antibody 11663-1-AP | Download protocol |
| WB protocol for VDAC1/2/3 antibody 11663-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Mitochondria regulate intracellular coenzyme Q transport and ferroptotic resistance via STARD7 | ||
Nat Cell Biol De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence | ||
Nat Cell Biol Leucine inhibits degradation of outer mitochondrial membrane proteins to adapt mitochondrial respiration. | ||
Nat Neurosci Mitochondrial accumulation and lysosomal dysfunction result in mitochondrial plaques in Alzheimer's disease | ||
Autophagy MYBL2 guides autophagy suppressor VDAC2 in the developing ovary to inhibit autophagy through a complex of VDAC2-BECN1-BCL2L1 in mammals. | ||
Autophagy Mitochondrial NAD(+)-mediated mitophagy alleviates type I interferon response to the cytosolic mitochondrial DNA. |