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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VDAC2 Recombinant antibody, PBS Only (Capture)

VDAC2 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241569G11

Cat no : 84225-2-PBS

Synonyms

VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569G11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VDAC2 Recombinant antibody (84225-2-RR)

    WB analysis using 84225-2-RR (same clone as 84225-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84225-2-RR (VDAC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using VDAC2 Recombinant antibody (84225-2-RR)

    FC experiment of HepG2 using 84225-2-RR (same clone as 84225-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-2-RR, Clone:241569G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01162-1

    Cytometric bead array standard curve of MP01162-1

    Cytometric bead array standard curve of MP01162-1, VDAC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84225-2-PBS. Detection antibody: 84225-1-PBS. Standard: Ag2266. Range: 1.563-200 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84225-2-PBS targets VDAC2 as part of a matched antibody pair:

MP01162-1: 84225-2-PBS capture and 84225-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen VDAC2 fusion protein Ag2266 相同性解析による交差性が予測される生物種
Full Name voltage-dependent anion channel 2
Calculated molecular weight 294 aa, 32 kDa
Observed molecular weight 31-33 kDa
GenBank accession numberBC000165
Gene symbol VDAC2
Gene ID (NCBI) 7417
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)