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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse heart tissue using VDAC2 Recombinant antibody (84225-3-RR)

    IHC staining of mouse heart using 84225-3-RR (same clone as 84225-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84225-3-RR (VDAC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using VDAC2 Recombinant antibody (84225-3-RR)

    IHC staining of mouse heart using 84225-3-RR (same clone as 84225-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84225-3-RR (VDAC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VDAC2 Recombinant antibody (84225-3-RR)

    IF Staining of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VDAC2 antibody (84225-3-RR, Clone: 241569C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using VDAC2 Recombinant antibody (84225-3-RR)

    FC experiment of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-3-RR, Clone:241569C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

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VDAC2 Recombinant antibody, PBS Only

VDAC2 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84225-3-PBS
Clone No.241569C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569C3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84225-3-PBS targets VDAC2 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen VDAC2 fusion protein Ag2266 相同性解析による交差性が予測される生物種
Full Name voltage-dependent anion channel 2
Calculated molecular weight 294 aa, 32 kDa
GenBank accession numberBC000165
Gene Symbol VDAC2
Gene ID (NCBI) 7417
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP45880
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)