Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue, human brain tissue, human heart tissue, mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, human osteosarcoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IHC | See 2 publications below |
Product Information
14451-1-AP targets VDAC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VDAC3 fusion protein Ag5960 相同性解析による交差性が予測される生物種 |
| Full Name | voltage-dependent anion channel 3 |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC056870 |
| Gene Symbol | VDAC3 |
| Gene ID (NCBI) | 7419 |
| RRID | AB_2212351 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y277 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VDAC3 antibody 14451-1-AP | Download protocol |
| IHC protocol for VDAC3 antibody 14451-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Mol Ther Nucleic Acids Salvia miltiorrhiza-Derived Sal-miR-58 Induces Autophagy and Attenuates Inflammation in Vascular Smooth Muscle Cells. | ||
Oncotarget Proteomics-based identification of VDAC1 as a tumor promoter in cervical carcinoma. | ||
J Cell Sci Doublet 7 shortening, doublet 5-preferential poly-Glu reduction, and beating stall of sperm flagella in Ttll9-/- mice. | ||
Phytomedicine Hedyotis diffusa injection induces ferroptosis via the Bax/Bcl2/VDAC2/3 axis in lung adenocarcinoma. | ||