Validation Data Gallery

Filter: AllWBIHC

WB analysis of human plasma using 16922-1-AP
human blood were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human blood using 16922-1-AP
human blood tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of rat eye using 16922-1-AP
rat eye tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (Vitamin D binding protein antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse eye using 16922-1-AP
mouse eye tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 16922-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse eye using 16922-1-AP
mouse eye tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 16922-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 16922-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16922-1-AP (Vitamin D binding protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 16922-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16922-1-AP (Vitamin D binding protein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 16922-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16922-1-AP (Vitamin D binding protein antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Vitamin D binding protein Polyclonal antibody

Vitamin D binding protein Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16922-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

GC, VDBP,GC, Gc protein-derived macrophage activating factor, Gc globulin, DBP-maf

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human blood tissue, human blood, rat eye tissue, mouse eye tissue, PC-3 cells, human plasma, mouse testis tissue
Positive IHC detected in	human liver tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 10 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

16922-1-AP targets Vitamin D binding protein in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Vitamin D binding protein fusion protein Ag10286 相同性解析による交差性が予測される生物種
Full Name	group-specific component (vitamin D binding protein)
Calculated molecular weight	474 aa, 53 kDa
Observed molecular weight	52-58 kDa
GenBank accession number	BC057228
Gene Symbol	Vitamin D binding protein
Gene ID (NCBI)	2638
RRID	AB_10597098
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P02774
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

Protocols

Product Specific Protocols
WB protocol for Vitamin D binding protein antibody 16922-1-AP	Download protocol
IHC protocol for Vitamin D binding protein antibody 16922-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Ther Methods Clin Dev Lentiviral vector mediated gene therapy for type I Dent disease ameliorates Dent disease-like phenotypes for three months in ClC-5 null mice Authors - Manish Kumar Yadav View Article
rat	WB	Sci Rep PMID: 31792309 Authors - Nela Pavlíková View Article
		Proteomics Screening for potential serum biomarkers in rat mesangial proliferative nephritis. Authors - Yang Lu View Article
rat	WB,IHC	Mol Med Rep Proteomics of acute heart failure in a rat post-myocardial infarction model. Authors - Yichen Guo View Article
mouse	WB	Pharmaceutics Evaluation of In Vitro and In Vivo Antiviral Activities of Vitamin D for SARS-CoV-2 and Variants Authors - Chee-Keng Mok View Article
mouse	IHC	Int Immunopharmacol Vitamin D attenuates DNCB-induced atopic dermatitis-like skin lesions by inhibiting immune response and restoring skin barrier function Authors - Renwei Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human plasma using 16922-1-AP

WB analysis of human blood using 16922-1-AP

WB analysis of rat eye using 16922-1-AP

WB analysis of mouse eye using 16922-1-AP

WB analysis of PC-3 using 16922-1-AP

WB analysis of mouse eye using 16922-1-AP

WB analysis of mouse testis using 16922-1-AP

IHC staining of human liver using 16922-1-AP

IHC staining of human liver using 16922-1-AP

IHC staining of human normal colon using 16922-1-AP

Vitamin D binding protein Polyclonal antibody

Vitamin D binding protein Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Lentiviral vector mediated gene therapy for type I Dent disease ameliorates Dent disease-like phenotypes for three months in ClC-5 null mice

PMID: 31792309

Screening for potential serum biomarkers in rat mesangial proliferative nephritis.

Proteomics of acute heart failure in a rat post-myocardial infarction model.

Evaluation of In Vitro and In Vivo Antiviral Activities of Vitamin D for SARS-CoV-2 and Variants

Vitamin D attenuates DNCB-induced atopic dermatitis-like skin lesions by inhibiting immune response and restoring skin barrier function