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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of bEnd.3 cells using VE-cadherin Recombinant antibody (84529-3-RR)

    IF Staining of bEnd.3 using 84529-3-RR

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using VE-cadherin antibody (84529-3-RR, Clone: 241953H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84529-3-RR

    Affinity and Kinetic Characterization of 84529-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84529-3-RR against Mouse VE-cadherin were performed. The affinity constant is 1.22 nM.

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VE-cadherin Recombinant antibody

VE-cadherin Recombinant Antibody for IF/ICC, ELISA
Cat No. 84529-3-RR
Clone No.241953H6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, ELISA

Synonyms

VE Cad, Cdh5, Cd144, cadherin 5, 7B4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inbEnd.3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84529-3-RR targets VE-cadherin in IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name cadherin 5
Calculated molecular weight88kDa
GenBank accession numberNM_009868.4
Gene Symbol Cdh5
Gene ID (NCBI) 12562
RRIDAB_3672037
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55284
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).

Protocols

Product Specific Protocols
IF protocol for VE-cadherin antibody 84529-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols