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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VE-cadherin/CD144 Polyclonal antibody

VE-cadherin/CD144 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31772-1-AP

Synonyms

VE-cadherin, Vascular endothelial cadherin, CDH5, CD144, Cadherin 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VE-cadherin/CD144 Polyclonal antibody (31772-1-AP)

    WB analysis using 31772-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31772-1-AP (VE-cadherin/CD144 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using VE-cadherin/CD144 Polyclonal antibody (31772-1-AP)

    IF Staining of HUVEC using 31772-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using VE-cadherin/CD144 antibody (31772-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inHUVEC cells, human placenta tissue
Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31772-1-AP targets VE-cadherin/CD144 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name cadherin 5, type 2 (vascular endothelium)
Calculated molecular weight88 kDa
Observed molecular weight90-100 kDa, 130-140 kDa
GenBank accession numberNM_001795.5
Gene symbol VE-cadherin
Gene ID (NCBI) 1003
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

Protocols

Product Specific Protocols
WB protocol for VE-cadherin/CD144 antibody 31772-1-APDownload protocol
IF protocol for VE-cadherin/CD144 antibody 31772-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols