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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VE-cadherin/CD144 Recombinant antibody, PBS Only

VE-cadherin/CD144 Recombinant Antibody for WB, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240755D5

Cat no : 83766-6-PBS

Synonyms

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VE-cadherin/CD144 Recombinant antibody (83766-6-RR)

    WB analysis using 83766-6-RR (same clone as 83766-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83766-6-RR (VE-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83766-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using VE-cadherin/CD144 Recombinant antibody (83766-6-RR)

    IF Staining of human placenta using 83766-6-RR (same clone as 83766-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using VE-cadherin/CD144 antibody (83766-6-RR, Clone: 240755D5 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83766-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83766-6-RR

    Affinity and Kinetic Characterization of 83766-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83766-6-RR against Human VE-cadherin/CD144 were performed. The affinity constant is 0.633 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83766-6-PBS is VE-cadherin/CD144 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name cadherin 5, type 2 (vascular endothelium)
Calculated molecular weight88 kDa
Observed molecular weight90-100 kDa, 130-140 kDa
GenBank accession numberNM_001795.5
Gene symbol VE-cadherin
Gene ID (NCBI) 1003
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).