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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VE-cadherin Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.78-100 ng/mL

Sandwich ELISA: 0.78-50 ng/mL

Cat No : MP00752-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00752-3, Human VE-cadherin Recombinant Matched Antibody Pair - PBS only. 83766-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1200. 83766-4-PBS was HRP conjugated as the detection antibody. Range: 0.78-50 ng/mL

    Sandwich ELISA standard curve of MP00752-3

    Sandwich ELISA standard curve of MP00752-3, Human VE-cadherin Recombinant Matched Antibody Pair - PBS only. 83766-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1200. 83766-4-PBS was HRP conjugated as the detection antibody. Range: 0.78-50 ng/mL

  • HUVEC were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human VE-cadherin, and measured 175.74 ng/mL. JAR were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human VE-cadherin, and measured 4.13 ng/mL.

    Sample test of MP00752-3

    HUVEC were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human VE-cadherin, and measured 175.74 ng/mL. JAR were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human VE-cadherin, and measured 4.13 ng/mL.

  • Serum of sixteen humans was measured. The human SIRPA concentration of detected samples was determined to be 3,148.2 ng/mL with a range of 2,070.2 - 4,839.1 pg/mL.

    Sample test of MP00752-3

    Serum of sixteen humans was measured. The human SIRPA concentration of detected samples was determined to be 3,148.2 ng/mL with a range of 2,070.2 - 4,839.1 pg/mL.

  • Cytometric bead array standard curve of MP00752-3, VE-cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83766-1-PBS. Detection antibody: 83766-4-PBS. Standard: Eg1200. Range: 0.78-100 ng/mL.

    Cytometric bead array standard curve of MP00752-3

    Cytometric bead array standard curve of MP00752-3, VE-cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83766-1-PBS. Detection antibody: 83766-4-PBS. Standard: Eg1200. Range: 0.78-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00752-3 targets VE-cadherin in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.78-100 ng/mL (Cytometric Bead Array); 0.78-50 ng/mL (Sandwich ELISA);

Capture antibody: VE-cadherin/CD144 Recombinant antibody, PBS Only (Capture) 83766-1-PBS (240755A6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: VE-cadherin/CD144 Recombinant antibody, PBS Only (Detector) 83766-4-PBS (240755F1). 100 μg. Concentration 1 mgl/ml.

Alternative VE-cadherin matched antibody pairs: MP00752-1, MP00752-2

Matched pair protein standard: Eg1200

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83766-1-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 5, type 2 (vascular endothelium)
Synonyms VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen
GenBank accession numberNM_001795.5
Gene symbol VE-cadherin
UniprotP33151-1
Gene ID (NCBI) 1003
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83766-4-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 5, type 2 (vascular endothelium)
Synonyms VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen
GenBank accession numberNM_001795.5
Gene symbol VE-cadherin
UniprotP33151-1
Gene ID (NCBI) 1003
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.