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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse VEGFA Recombinant antibody, PBS Only (Detector)

VEGFA Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241391F2

Cat no : 84093-5-PBS

Synonyms

Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF, L-VEGF

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00561-4

    Sandwich ELISA standard curve of MP00561-4

    Sandwich ELISA standard curve of MP00561-4, Mouse VEGF Recombinant Matched Antibody Pair - PBS only. 84093-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0278. 84093-5-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 pg/mL

  • Sample test of MP00561-4

    Sample test of MP00561-4

    Mouse lung tissue was rinsed with 1X PBS to remove excess blood, homogenized in 20 mL of 1X PBS and stored overnight at ≤ -20 °C. Homogenates were thawed and centrifuged for 5 minutes at 5000 x g. Homogenates were assayed for mouse VEGFA120 and measured 775.6 pg/mL based on a 1.8 mg/mL extract load.

  • Sample test of MP00561-4

    Sample test of MP00561-4

    Serum of eight mice was measured. The mouse VEGFA120 concentration of detected samples was determined to be 12.6 pg/mL with a range of 7.3 - 19.7 pg/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84093-5-PBS targets VEGFA as part of a matched antibody pair:

MP00561-4: 84093-2-PBS capture and 84093-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name vascular endothelial growth factor A
Calculated molecular weight17kd
GenBank accession numberNM_001287058
Gene symbol Vegfa
Gene ID (NCBI) 22339
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.