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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VEGFR-1/FLT-1 Polyclonal antibody, PBS Only

VEGFR-1/FLT-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13687-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using VEGFR-1/FLT-1 Polyclonal antibody (13687-1-AP)

    WB analysis of HEK-293 using 13687-1-AP (same clone as 13687-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using VEGFR-1/FLT-1 Polyclonal antibody (13687-1-AP)

    WB analysis of human placenta using 13687-1-AP (same clone as 13687-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using VEGFR-1/FLT-1 Polyclonal antibody (13687-1-AP)

    WB analysis of mouse lung using 13687-1-AP (same clone as 13687-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A549 cells using VEGFR-1/FLT-1 Polyclonal antibody (13687-1-AP)

    IP experiment of A549 using 13687-1-AP (same clone as 13687-1-PBS)

    IP result of anti-VEGFR-1/FLT-1 (IP:13687-1-AP, 5ug; Detection:13687-1-AP 1:500) with A549 cells lysate 3300ug. This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using VEGFR-1/FLT-1 Polyclonal antibody (13687-1-AP)

    IHC staining of human renal cell carcinoma using 13687-1-AP (same clone as 13687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using VEGFR-1/FLT-1 Polyclonal antibody (13687-1-AP)

    IHC staining of human renal cell carcinoma using 13687-1-AP (same clone as 13687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VEGFR-1/FLT-1 Polyclonal antibody (13687-1-AP)

    IF Staining of HeLa using 13687-1-AP (same clone as 13687-1-PBS)

    Immunofluorescent analysis of HeLa cells using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13687-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13687-1-PBS targets VEGFR-1/FLT-1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VEGFR-1/FLT-1 fusion protein Ag4558 相同性解析による交差性が予測される生物種
Full Name fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
Calculated molecular weight 1338 aa, 151 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC039007
Gene symbol VEGFR-1/FLT-1
Gene ID (NCBI) 2321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders, and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF, and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Two isoforms of VEGFR-1 exist, a full-length transmembrane form and a short soluble form (sVEGFR-1) consisting of only the extracellular ligand-binding domain. (PMID: 12778165; 17109193; 8806634)