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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse VEGFR2/CD309 Rabbit Recombinant Antibody

VEGFR2/CD309 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240214F12

Cat no : 98027-1-RR

Synonyms

Kdr, Krd 1, Flk1, Flk 1, 6130401C07

Validation Data Gallery

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  • Flow cytometry (FC) experiment of bEnd.3 cells using Anti-Mouse VEGFR2/CD309 Rabbit Recombinant Antibod (98027-1-RR)

    FC experiment of bEnd.3 using 98027-1-RR

    1x10^6 bEnd.3 cells were surface stained with 0.25 ug Anti-Mouse VEGFR2/CD309 Rabbit Recombinant Antibody (98027-1-RR, Clone: 240214F12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98027-1-RR

    Affinity and Kinetic Characterization of 98027-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98027-1-RR against Mouse VEGFR-2/Kdr were performed. The affinity constant is 2.92 nM.

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Tested Applications

Positive FC detected inbEnd.3 cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98027-1-RR is VEGFR2/CD309 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name kinase insert domain protein receptor
Calculated molecular weight150KD
GenBank accession numberNM_010612.2
Gene symbol VEGFR-2
Gene ID (NCBI) 16542
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

VEGFR2, also named as CD309, KDR and FLK1, is a receptor for VEGF or VEGFC. VEGFR2 which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. VEGFR2 functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Protocols

Product Specific Protocols
FC protocol for VEGFR2/CD309 antibody 98027-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols