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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VEGFR2/KDR Recombinant antibody

VEGFR2/KDR Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240214D7

Cat no : 83366-1-RR

Synonyms

Kdr, Krd 1, Flk1, Flk 1, 6130401C07

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using VEGFR2/KDR Recombinant antibody (83366-1-RR)

    WB analysis of mouse lung using 83366-1-RR

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 83366-1-RR (VEGFR2/KDR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83366-1-RR

    Affinity and Kinetic Characterization of 83366-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83366-1-RR against Mouse VEGFR2/KDR were performed. The affinity constant is 1.88 nM.

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Tested Applications

Positive WB detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83366-1-RR is VEGFR2/KDR Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name kinase insert domain protein receptor
Calculated molecular weight150KD
Observed molecular weight150-190 kDa
GenBank accession numberNM_010612.2
Gene symbol VEGFR-2
Gene ID (NCBI) 16542
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VEGFR2, also named CD309, KDR, and FLK1, is a receptor for VEGF or VEGFC. VEGFR2 which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In the case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. VEGFR2 functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis, and sprouting.

Protocols

Product Specific Protocols
WB protocol for VEGFR2/KDR antibody 83366-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols