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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SLC32A1/VGAT Polyclonal antibody (14471-1-AP)

    WB analysis of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 14471-1-AP (VGAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SLC32A1/VGAT Polyclonal antibody (14471-1-AP)

    IP experiment of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    IP result of anti-VGAT (IP:14471-1-AP, 4ug; Detection:14471-1-AP 1:5000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SLC32A1/VGAT Polyclonal antibody (14471-1-AP)

    IHC staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC32A1/VGAT Polyclonal antibody (14471-1-AP)

    IHC staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using SLC32A1/VGAT Polyclonal antibody (14471-1-AP)

    IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SLC32A1/VGAT Polyclonal antibody (14471-1-AP)

    IF Staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using SLC32A1/VGAT Polyclonal antibody (14471-1-AP)

    IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using SLC32A1/VGAT Polyclonal antibody (14471-1-AP)

    IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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SLC32A1/VGAT Polyclonal antibody, PBS Only

SLC32A1/VGAT Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 14471-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

SLC32A1, VGAT, VIAAT, Vesicular GABA transporter, hVIAAT

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14471-1-PBS targets SLC32A1/VGAT in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC32A1/VGAT fusion protein Ag5843 相同性解析による交差性が予測される生物種
Full Name solute carrier family 32 (GABA vesicular transporter), member 1
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC053582
Gene Symbol VGAT
Gene ID (NCBI) 140679
RRIDAB_10644324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H598
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.