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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VGAT Polyclonal antibody, PBS Only

VGAT Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14471-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using VGAT Polyclonal antibody (14471-1-AP)

    WB analysis of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 14471-1-AP (VGAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using VGAT Polyclonal antibody (14471-1-AP)

    IP experiment of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    IP result of anti-VGAT (IP:14471-1-AP, 4ug; Detection:14471-1-AP 1:5000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using VGAT Polyclonal antibody (14471-1-AP)

    IHC staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VGAT Polyclonal antibody (14471-1-AP)

    IHC staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using VGAT Polyclonal antibody (14471-1-AP)

    IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using VGAT Polyclonal antibody (14471-1-AP)

    IF Staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using VGAT Polyclonal antibody (14471-1-AP)

    IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using VGAT Polyclonal antibody (14471-1-AP)

    IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14471-1-PBS targets VGAT in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VGAT fusion protein Ag5843 相同性解析による交差性が予測される生物種
Full Name solute carrier family 32 (GABA vesicular transporter), member 1
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC053582
Gene symbol VGAT
Gene ID (NCBI) 140679
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.