Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, human brain tissue, rat brain tissue, PC-3 cells, mouse brain tissue, mouse spleen tissue, rat spleen tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
10124-2-AP targets VGLL1 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VGLL1 fusion protein Ag0169 相同性解析による交差性が予測される生物種 |
| Full Name | vestigial like 1 (Drosophila) |
| Calculated molecular weight | 29 kDa |
| Observed molecular weight | 26-29 kDa |
| GenBank accession number | BC000045 |
| Gene Symbol | VGLL1 |
| Gene ID (NCBI) | 51442 |
| RRID | AB_2218174 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99990 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VGLL1 is a specific coactivator for the mammalian TEFs. It can bind proteins of the TEA domain family of transcription factors. Vgll1-TEFs complex upregulates the expression of IGFBP-5, a proliferation-promoting gene, and facilitates anchorage-independent cell proliferation. The calculated molecular weight of VGLL1 is 29 kDa, and the observed molecular weight is 26-29 kDa(PMID:31816819,33087697).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VGLL1 antibody 10124-2-AP | Download protocol |
| IHC protocol for VGLL1 antibody 10124-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Endocr Relat Cancer Vgll1 expression is associated with a triple-negative basal-like phenotype in breast cancer. | ||
Cancers (Basel) PI3K/AKT/β-Catenin Signaling Regulates Vestigial-Like 1 Which Predicts Poor Prognosis and Enhances Malignant Phenotype in Gastric Cancer.
| ||
Int J Mol Sci TNF−α Secreted from Macrophages Increases the Expression of Prometastatic Integrin αV in Gastric Cancer
| ||
iScience N6-methyladenosine-modified VGLL1 promotes ovarian cancer metastasis through high-mobility group AT-hook 1/Wnt/β-catenin signaling | ||
Sci Rep A novel signature constructed by mitochondrial function and cell death-related gene for the prediction of prognosis in bladder cancer | ||
Front Oncol Placental co-transcriptional activator Vestigial-like 1 (VGLL1) drives tumorigenesis via increasing transcription of proliferation and invasion genes |