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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VGLUT2 Polyclonal antibody (25261-1-AP)

    WB analysis using 25261-1-AP

    unboiled rat brain tissue were subjected to SDS PAGE followed by western blot with 25261-1-AP (VGLUT2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using VGLUT2 Polyclonal antibody (25261-1-AP)

    WB analysis of fetal human brain using 25261-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25261-1-AP (VGLUT2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

VGLUT2 Polyclonal antibody

VGLUT2 Polyclonal Antibody for WB, ELISA
Cat No. 25261-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

SLC17A6, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, DNPI

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inunboiled rat brain tissue, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25261-1-AP targets VGLUT2 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Calculated molecular weight 64 kDa
Observed molecular weight 60 kDa
GenBank accession numberNM_020346
Gene Symbol VGLUT2
Gene ID (NCBI) 57084
RRIDAB_2879992
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2U8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicle (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).

Protocols

Product Specific Protocols
WB protocol for VGLUT2 antibody 25261-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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