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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VGLUT2 Polyclonal antibody (29209-1-AP)

    WB analysis using 29209-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29209-1-AP (VGLUT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VGLUT2 Polyclonal antibody (29209-1-AP)

    IHC staining of mouse brain using 29209-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29209-1-AP (VGLUT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VGLUT2 Polyclonal antibody (29209-1-AP)

    IHC staining of mouse brain using 29209-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29209-1-AP (VGLUT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using VGLUT2 Polyclonal antibody (29209-1-AP)

    IF Staining of mouse brain using 29209-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGLUT2 antibody (29209-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using VGLUT2 Polyclonal antibody (29209-1-AP)

    IF Staining of mouse brain using 29209-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGLUT2 antibody (29209-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using VGLUT2 Polyclonal antibody (29209-1-AP)

    IF Staining of mouse brain using 29209-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using VGLUT2 antibody (29209-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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VGLUT2 Polyclonal antibody

VGLUT2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 29209-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

SLC17A6, DNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Differentiation-associated BNPI

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inunboiled mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29209-1-AP targets VGLUT2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VGLUT2 fusion protein Ag29565 相同性解析による交差性が予測される生物種
Full Name solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Calculated molecular weight 64 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberNM_020346
Gene Symbol VGLUT2
Gene ID (NCBI) 57084
RRIDAB_3086104
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2U8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicles (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).

Protocols

Product Specific Protocols
WB protocol for VGLUT2 antibody 29209-1-APDownload protocol
IHC protocol for VGLUT2 antibody 29209-1-APDownload protocol
IF protocol for VGLUT2 antibody 29209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols