VGLUT2 Polyclonal antibody
VGLUT2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 29209-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | unboiled mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
29209-1-AP targets VGLUT2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | VGLUT2 fusion protein Ag29565 相同性解析による交差性が予測される生物種 |
Full Name | solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 |
Calculated molecular weight | 64 kDa |
Observed molecular weight | 60-70 kDa |
GenBank accession number | NM_020346 |
Gene symbol | VGLUT2 |
Gene ID (NCBI) | 57084 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicles (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).
Protocols
Product Specific Protocols | |
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WB protocol for VGLUT2 antibody 29209-1-AP | Download protocol |
IHC protocol for VGLUT2 antibody 29209-1-AP | Download protocol |
IF protocol for VGLUT2 antibody 29209-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |