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KD/KO Validated

Vimentin Polyclonal antibody

Name: Vimentin Polyclonal antibody
Brand: Proteintech
SKU: 22031-1-AP

Vimentin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22031-1-AP

Synonyms

VIM

Validation Data Gallery

Filter:

AllWBIHCIF-P

WB analysis of HEK-293 using 22031-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22031-1-AP (VIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 22031-1-AP
WB result of Vimentin antibody (22031-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Vimentin transfected A549 cells.
WB analysis of HeLa using 22031-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22031-1-AP (VIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 22031-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 22031-1-AP (VIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 22031-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22031-1-AP (Vimentin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 22031-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22031-1-AP (Vimentin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 22031-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22031-1-AP (Vimentin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 22031-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22031-1-AP (Vimentin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human kidney using 22031-1-AP
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (22031-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human kidney using 22031-1-AP
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (22031-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 22031-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, mouse heart tissue, A549 cells
Positive IHC detected in	human ovary tumor tissue, human tonsillitis tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human kidney tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IHC	See 1 publications below
IF	See 5 publications below

Product Information

22031-1-AP targets Vimentin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Vimentin fusion protein Ag16898 相同性解析による交差性が予測される生物種
Full Name	vimentin
Calculated molecular weight	466 aa, 54 kDa
Observed molecular weight	57 kDa
GenBank accession number	BC000163
Gene symbol	VIM
Gene ID (NCBI)	7431
RRID	AB_11182825
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Protocols

Product Specific Protocols
WB protocol for Vimentin antibody 22031-1-AP	Download protocol
IHC protocol for Vimentin antibody 22031-1-AP	Download protocol
IF protocol for Vimentin antibody 22031-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Tissue Eng Part C Methods Pretreatment with Inflammatory Factors Altered the Secretome of Human Amniotic Epithelial Cells Authors - Wenjiao Cao View Article
human	WB	Dig Liver Dis CTHRC1 serves as an indicator in biliary atresia for evaluating the stage of liver fibrosis and predicting prognosis Authors - Zequan Ding View Article
human	WB	Int J Clin Exp Pathol miR-34b-5p suppresses the epithelial-mesenchymal transition and metastasis in endometrial cancer AN3CA cells by targeting ZEB1 Authors - Lulu Shi View Article
mouse	IF	BMC Oral Health Fibrin-konjac glucomannan-black phosphorus hydrogel scaffolds loaded with nasal ectodermal mesenchymal stem cells accelerated alveolar bone regeneration Authors - Yin Zou View Article
human	WB,IF	Am J Cancer Res High glucose-induced NCAPD2 upregulation promotes malignant phenotypes and regulates EMT via the Wnt/β-catenin signaling pathway in HCC Authors - Yuhua Mai View Article
human	IF	Acta Biochim Biophys Sin (Shanghai) Integrated network pharmacology and experimental verification to explore the potential mechanism of San Ying decoction for treating triple-negative breast cancer Authors - Xiaojuan Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Vimentin Polyclonal antibody

Vimentin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 22031-1-AP

WB analysis of A549 using 22031-1-AP

WB analysis of HeLa using 22031-1-AP

WB analysis of mouse heart using 22031-1-AP

IHC staining of human ovary tumor using 22031-1-AP

IHC staining of human colon using 22031-1-AP

IHC staining of human tonsillitis using 22031-1-AP

IHC staining of human tonsillitis using 22031-1-AP

IF Staining of human kidney using 22031-1-AP

IF Staining of human kidney using 22031-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pretreatment with Inflammatory Factors Altered the Secretome of Human Amniotic Epithelial Cells

CTHRC1 serves as an indicator in biliary atresia for evaluating the stage of liver fibrosis and predicting prognosis

miR-34b-5p suppresses the epithelial-mesenchymal transition and metastasis in endometrial cancer AN3CA cells by targeting ZEB1

Fibrin-konjac glucomannan-black phosphorus hydrogel scaffolds loaded with nasal ectodermal mesenchymal stem cells accelerated alveolar bone regeneration

High glucose-induced NCAPD2 upregulation promotes malignant phenotypes and regulates EMT via the Wnt/β-catenin signaling pathway in HCC

Integrated network pharmacology and experimental verification to explore the potential mechanism of San Ying decoction for treating triple-negative breast cancer