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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VISTA Polyclonal antibody

VISTA Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 31619-1-AP

Synonyms

C10orf54, B7-H5, Sisp-1, SISP1, PP2135

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VISTA Polyclonal antibody (31619-1-AP)

    WB analysis using 31619-1-AP

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 31619-1-AP (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VISTA Polyclonal antibody (31619-1-AP)

    IHC staining of human tonsillitis using 31619-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31619-1-AP (VISTA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VISTA Polyclonal antibody (31619-1-AP)

    IHC staining of human tonsillitis using 31619-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31619-1-AP (VISTA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using VISTA Polyclonal antibody (31619-1-AP)

    IF Staining of human tonsillitis using 31619-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (31619-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman peripheral blood platelets
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31619-1-AP targets VISTA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 54
Calculated molecular weight 311 aa, 34 kDa
Observed molecular weight55 kDa
GenBank accession numberBC020568
Gene symbol VISTA
Gene ID (NCBI) 64115
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.

Protocols

Product Specific Protocols
WB protocol for VISTA antibody 31619-1-APDownload protocol
IHC protocol for VISTA antibody 31619-1-APDownload protocol
IF protocol for VISTA antibody 31619-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols