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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human VISTA Rabbit Recombinant Antibody, PBS Only

VISTA Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241003C8

Cat no : 98109-1-PBS

Synonyms

C10orf54, SISP1, PP2135, GI24, B7-H5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human VISTA Rabbit Recombinant Antibody (98109-1-RR)

    FC experiment of human peripheral blood leukocytes using 98109-1-RR (same clone as 98109-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human VISTA Rabbit Recombinant Antibody (98109-1-RR, Clone:241003C8) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98109-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human VISTA Rabbit Recombinant Antibody (98109-1-RR)

    FC experiment of human peripheral blood leukocytes using 98109-1-RR (same clone as 98109-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human VISTA Rabbit Recombinant Antibody (98109-1-RR, Clone:241003C8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98109-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98109-1-RR

    Affinity and Kinetic Characterization of 98109-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98109-1-RR against Human VISTA were performed. The affinity constant is 0.933 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98109-1-PBS is VISTA Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 54
Calculated molecular weight 311 aa, 34 kDa
GenBank accession numberBC020568
Gene symbol VISTA
Gene ID (NCBI) 64115
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.