Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 10 publications below |
| IF | See 1 publications below |
Product Information
19493-1-AP targets VLDLR in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, chicken, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | very low density lipoprotein receptor |
| Calculated molecular weight | 96 kDa |
| Observed molecular weight | 96 kDa |
| GenBank accession number | NM_003383 |
| Gene Symbol | VLDLR |
| Gene ID (NCBI) | 7436 |
| RRID | AB_10644342 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P98155 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VLDLR binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. VLDLR binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation (cytoplasmic domain of low-density lipoprotein receptor). For glycosylated, the MW of VLDLR migrates to ~160kd.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VLDLR antibody 19493-1-AP | Download protocol |
| IF protocol for VLDLR antibody 19493-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron Molecular microcircuitry underlies functional specification in a basal ganglia circuit dedicated to vocal learning. | ||
Food Funct Monounsaturated fatty acid-enriched olive oil exacerbates chronic alcohol-induced hepatic steatosis and liver injury in C57BL/6J mice | ||
Front Pharmacol Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats. | ||
PLoS One Gut microbiota and butyrate contribute to nonalcoholic fatty liver disease in premenopause due to estrogen deficiency. | ||
Front Nutr Dietary camellia seed oil attenuates liver injury in mice chronically exposed to alcohol | ||
Front Oncol VLDLR disturbs quiescence of breast cancer stem cells in a ligand-independent function
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