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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VLGR1 Polyclonal antibody (31628-1-AP)

    WB analysis using 31628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31628-1-AP (VLGR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VLGR1 Polyclonal antibody (31628-1-AP)

    WB analysis using 31628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31628-1-AP (VLGR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using VLGR1 Polyclonal antibody (31628-1-AP)

    IP experiment of SH-SY5Y using 31628-1-AP

    IP result of anti-VLGR1 (IP:31628-1-AP, 4ug; Detection:31628-1-AP 1:500) with SH-SY5Y cells lysate 1920 ug.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using VLGR1 Polyclonal antibody (31628-1-AP)

    IF Staining of K-562 using 31628-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using VLGR1 antibody (31628-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

VLGR1 Polyclonal antibody

VLGR1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 31628-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

GPR98, USH2B, MASS1, KIAA0686, G protein coupled receptor 98

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31628-1-AP targets VLGR1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VLGR1 fusion protein Ag34635 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 98
Calculated molecular weight693 kDa
Observed molecular weight217 kDa
GenBank accession numberNM_032119
Gene Symbol GPR98
Gene ID (NCBI) 84059
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8WXG9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VLGR1 (very large G protein-coupled receptor-1), also known as ADGRV1, GPR98, and MASS, has a molecular weight of up to 700 kDa, making it the largest member of the 33 adhesive G protein-coupled receptors (ADGRs), a unique subfamily of the GPCR superfamily. The full-length VLGR1 encompasses ∼6300 amino acids and thus is difficult to analyze by electrophoresis. Vlgr1 is a core component of ankle-link complex in inner ear hair cells. Knock-out and mutation mouse models show that loss of Vlgr1 function leads to abnormal stereociliary development and hearing loss, indicating crucial roles of Vlgr1 in hearing transduction or auditory system development (PMID: 23180093). Western blot analysis detected VLGR1a isoform at an apparent molecular mass of 217 kDa .

Protocols

Product Specific Protocols
WB protocol for VLGR1 antibody 31628-1-APDownload protocol
IF protocol for VLGR1 antibody 31628-1-APDownload protocol
IP protocol for VLGR1 antibody 31628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols