Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HEK-293 using 11612-1-AP
WB result of VPRBP antibody (11612-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPRBP transfected HEK-293 cells.
WB analysis using 11612-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 11612-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11612-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11612-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 11612-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11612-1-AP
IP result of anti-VPRBP (IP:11612-1-AP, 3ug; Detection:11612-1-AP 1:1000) with HeLa cells lysate 3800ug.
IHC staining of human prostate cancer using 11612-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11612-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 11612-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 11612-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11612-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11612-1-AP (VPRBP antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 11612-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (11612-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 11612-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug VPRBP Polyclonal antibody (11612-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

VPRBP Polyclonal antibody

VPRBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11612-1-AP

Publications(42)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

KIAA0800, HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (42)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	DU 145 cells, HeLa cells, mouse testis tissue, PC-3 cells, HEK-293 cells, K-562 cells, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human prostate cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 20 publications below
WB	See 38 publications below
IHC	See 7 publications below
IF	See 6 publications below
IP	See 4 publications below

Product Information

11612-1-AP targets VPRBP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VPRBP fusion protein Ag2184 相同性解析による交差性が予測される生物種
Full Name	Vpr (HIV-1) binding protein
Calculated molecular weight	1506 aa, 169 kDa
Observed molecular weight	169 kDa
GenBank accession number	BC022792
Gene Symbol	VPRBP
Gene ID (NCBI)	9730
RRID	AB_2216933
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y4B6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).

Protocols

Product Specific Protocols
WB protocol for VPRBP antibody 11612-1-AP	Download protocol
IHC protocol for VPRBP antibody 11612-1-AP	Download protocol
IF protocol for VPRBP antibody 11612-1-AP	Download protocol
IP protocol for VPRBP antibody 11612-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,IF	Science CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins. Authors - Chao Yu KD Validated View Article
mouse, human	WB, IF	Mol Cell CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET Dioxygenases. Authors - Tadashi Nakagawa KD Validated View Article
human	WB	Mol Cell Vpr Targets TET2 for Degradation by CRL4VprBP E3 Ligase to Sustain IL-6 Expression and Enhance HIV-1 Replication. Authors - Lei Lv KD Validated View Article
human	WB	Nat Microbiol Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex. Authors - Leonid Yurkovetskiy View Article
human	WB	Cell Host Microbe Vpr overcomes macrophage-specific restriction of HIV-1 Env expression and virion production. Authors - Michael Mashiba View Article
human	WB, IF	Cell Host Microbe Cyclin L2 Is a Critical HIV Dependency Factor in Macrophages that Controls SAMHD1 Abundance. Authors - George Boateng Kyei KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 11612-1-AP

WB analysis using 11612-1-AP

WB analysis of mouse testis using 11612-1-AP

WB analysis of HeLa using 11612-1-AP

WB analysis of K-562 using 11612-1-AP

WB analysis of PC-3 using 11612-1-AP

IP experiment of HeLa using 11612-1-AP

IHC staining of human prostate cancer using 11612-1-AP

IHC staining of human prostate cancer using 11612-1-AP

IHC staining of mouse testis using 11612-1-AP

IHC staining of mouse testis using 11612-1-AP

IHC staining of human prostate cancer using 11612-1-AP

IF Staining of HeLa using 11612-1-AP

FC experiment of HeLa using 11612-1-AP

VPRBP Polyclonal antibody

VPRBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.

CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET Dioxygenases.

Vpr Targets TET2 for Degradation by CRL4VprBP E3 Ligase to Sustain IL-6 Expression and Enhance HIV-1 Replication.

Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.

Vpr overcomes macrophage-specific restriction of HIV-1 Env expression and virion production.

Cyclin L2 Is a Critical HIV Dependency Factor in Macrophages that Controls SAMHD1 Abundance.