VPRBP Recombinant antibody, PBS Only (Capture)
VPRBP Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, Cytometric bead array, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
241028B6
Cat no : 83901-3-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
83901-3-PBS targets VPRBP as part of a matched antibody pair:
MP00852-2: 83901-3-PBS capture and 83901-4-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | VPRBP fusion protein Ag2184 相同性解析による交差性が予測される生物種 |
| Full Name | Vpr (HIV-1) binding protein |
| Calculated molecular weight | 1506 aa, 169 kDa |
| Observed molecular weight | 169 kDa |
| GenBank accession number | BC022792 |
| Gene symbol | VPRBP |
| Gene ID (NCBI) | 9730 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507 amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).
