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KD/KO Validated

VPS11 Polyclonal antibody

Name: VPS11 Polyclonal antibody
Brand: Proteintech
SKU: 19140-1-AP

VPS11 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), IP, ELISA, IF

Conjugate

Unconjugated

Publications

Review Product

Cat no : 19140-1-AP

Synonyms

Vacuolar protein sorting-associated protein 11 homolog, RNF108, RING finger protein 108, PP3476, hVPS11

Validation Data Gallery

Filter:

AllWBIPFC (Intra)

WB analysis using 19140-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 19140-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 19140-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 19140-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 19140-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19140-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 19140-1-AP
IP result of anti-VPS11 (IP:19140-1-AP, 4ug; Detection:19140-1-AP 1:500) with HEK-293 cells lysate 1480ug.
FC experiment of HepG2 using 19140-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug VPS11 Polyclonal antibody (19140-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 19140-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, human brain tissue, mouse pancreas tissue, mouse brain tissue, human kidney tissue, human heart tissue, HeLa cells, K-562 cells, rat brain tissue
Positive IP detected in	HEK-293 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

19140-1-AP targets VPS11 in WB, FC (Intra), IP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VPS11 fusion protein Ag6227 相同性解析による交差性が予測される生物種
Full Name	vacuolar protein sorting 11 homolog (S. cerevisiae)
Calculated molecular weight	108 kDa
Observed molecular weight	108-112 kDa
GenBank accession number	BC065563
Gene symbol	VPS11
Gene ID (NCBI)	55823
RRID	AB_10642572
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Vesicle-mediated protein sorting plays an important role in segregating intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. VPS11 is the human homolog of the yeast class C Vps11 protein, a subunit of the HOPS (homotypic fusion and protein transport) complex. Mammalian Vps11 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and membrane docking/fusion reactions of late endosomes/lysosomes.

Protocols

Product Specific Protocols
WB protocol for VPS11 antibody 19140-1-AP	Download protocol
IP protocol for VPS11 antibody 19140-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes Authors - Wang Zheng View Article
human	WB,IF	J Cell Sci Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L. Authors - van der Kant Rik R KO Validated View Article
human	WB	J Mol Biol Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B. Authors - Morag R Hunter View Article
human	WB, IF	J Biol Chem Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity. Authors - Rik van der Kant View Article
human	IF,WB	J Cell Sci The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome. Authors - Magdalena Banach-Orłowska KD Validated View Article
human	WB,IP	Biochem J VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction. Authors - Morag R Hunter View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS11 Polyclonal antibody

VPS11 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 19140-1-AP

WB analysis of human brain using 19140-1-AP

WB analysis of mouse pancreas using 19140-1-AP

WB analysis of human kidney using 19140-1-AP

WB analysis of human heart using 19140-1-AP

WB analysis of mouse brain using 19140-1-AP

IP experiment of HEK-293 using 19140-1-AP

FC experiment of HepG2 using 19140-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.

Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity.

The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome.

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.