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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS13A Polyclonal antibody

VPS13A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28618-1-AP

Synonyms

CHAC, Chorea acanthocytosis protein, CHOREIN, FLJ42030, KIAA0986, VPS13A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS13A Polyclonal antibody (28618-1-AP)

    WB analysis using 28618-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28618-1-AP (VPS13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VPS13A Polyclonal antibody (28618-1-AP)

    WB analysis using 28618-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28618-1-AP (VPS13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS13A Polyclonal antibody (28618-1-AP)

    IHC staining of mouse brain using 28618-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS13A Polyclonal antibody (28618-1-AP)

    IHC staining of mouse brain using 28618-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using VPS13A Polyclonal antibody (28618-1-AP)

    IHC staining of human kidney using 28618-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using VPS13A Polyclonal antibody (28618-1-AP)

    IHC staining of human kidney using 28618-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, mouse heart tissue
Positive IHC detected inmouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28618-1-AP targets VPS13A in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS13A fusion protein Ag29532 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 13 homolog A (S. cerevisiae)
Calculated molecular weight 360 kDa
Observed molecular weight360 kDa
GenBank accession numberNM_033305
Gene symbol VPS13A
Gene ID (NCBI) 23230
RRIDAB_2918183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).

Protocols

Product Specific Protocols
WB protocol for VPS13A antibody 28618-1-APDownload protocol
IHC protocol for VPS13A antibody 28618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Analysis of Brain, Blood, and Testis Phenotypes Lacking the Vps13a Gene in C57BL/6N Mice

Authors - Jitrapa Pinyomahakul
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tom (Verified Customer) (09-27-2021)

Lysate from HEK293T - detects endogenous protein.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
VPS13A Antibody Western Blot validation (1:500 dilution) in (Cat no:28618-1-AP)