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VPS13C Polyclonal antibody
VPS13C Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 28676-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 4 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
28676-1-AP targets VPS13C in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | VPS13C fusion protein Ag29434 相同性解析による交差性が予測される生物種 |
Full Name | vacuolar protein sorting 13 homolog C (S. cerevisiae) |
Observed molecular weight | 422 kDa |
GenBank accession number | NM_001018088 |
Gene symbol | VPS13C |
Gene ID (NCBI) | 54832 |
RRID | AB_2881191 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson's disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).
Protocols
Product Specific Protocols | |
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WB protocol for VPS13C antibody 28676-1-AP | Download protocol |
IHC protocol for VPS13C antibody 28676-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Biol ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling. | ||
iScience Downregulation of VPS13C promotes cisplatin resistance in cervical cancer by upregulating GSTP1
| ||
J Cell Biol VPS13C regulates phospho-Rab10-mediated lysosomal function in human dopaminergic neurons | ||
Biomedicines A Pilot Study on the Proteomics Profile of Serum Exosome-Enriched Extracellular Vesicles from Normal versus Individuals with Obesity-Related Insulin Resistance | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tom (Verified Customer) (01-25-2021) | HEK293T lysates (10ug) ran on 4-12% gradient gel prior to transfer to nitrocellulose membrane. Membrane incubated overnight at 4 degrees with antibody (1:250). Goat anti-rabbit HRP secondary antibody (1:10,000) incubated with membrane for 1 hour at RT.
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