• Featured Product
  • KD/KO Validated

VPS13C Polyclonal antibody

VPS13C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28676-1-AP

Synonyms


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Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28676-1-AP targets VPS13C in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS13C fusion protein Ag29434 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 13 homolog C (S. cerevisiae)
Observed molecular weight 422 kDa
GenBank accession numberNM_001018088
Gene symbol VPS13C
Gene ID (NCBI) 54832
RRIDAB_2881191
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson's disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).

Protocols

Product Specific Protocols
WB protocol for VPS13C antibody 28676-1-APDownload protocol
IHC protocol for VPS13C antibody 28676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Biol

ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling.

Authors - William Hancock-Cerutti
human,mouseWB,IHC,IF

iScience

Downregulation of VPS13C promotes cisplatin resistance in cervical cancer by upregulating GSTP1

Authors - Xiangyu Tan
  • KD Validated
human

J Cell Biol

VPS13C regulates phospho-Rab10-mediated lysosomal function in human dopaminergic neurons

Authors - Leonie F Schrӧder
humanWB

Biomedicines

A Pilot Study on the Proteomics Profile of Serum Exosome-Enriched Extracellular Vesicles from Normal versus Individuals with Obesity-Related Insulin Resistance

Authors - Viswanathan Saraswathi
humanWB

Contact (Thousand Oaks)

VPS13A and VPS13C Influence Lipid Droplet Abundance

Authors - Shuliang Chen
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (01-25-2021)

HEK293T lysates (10ug) ran on 4-12% gradient gel prior to transfer to nitrocellulose membrane. Membrane incubated overnight at 4 degrees with antibody (1:250). Goat anti-rabbit HRP secondary antibody (1:10,000) incubated with membrane for 1 hour at RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: HEK293T
VPS13C Antibody Western Blot validation (1:250 dilution) in HEK293T (Cat no:28676-1-AP)