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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS15 Polyclonal antibody, PBS Only

VPS15 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17894-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using VPS15 Polyclonal antibody (17894-1-AP)

    WB analysis of HepG2 using 17894-1-AP (same clone as 17894-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17894-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VPS15 Polyclonal antibody (17894-1-AP)

    WB analysis using 17894-1-AP (same clone as 17894-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17894-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VPS15 Polyclonal antibody (17894-1-AP)

    IF Staining of HeLa using 17894-1-AP (same clone as 17894-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VPS15 antibody (17894-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 17894-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17894-1-PBS targets VPS15 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS15 fusion protein Ag12221 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, regulatory subunit 4
Calculated molecular weight 1358 aa, 153 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC110318
Gene symbol PIK3R4
Gene ID (NCBI) 30849
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PIK3R4(phosphoinositide 3-kinase regulatory subunit 4) is also named as PI3-kinase p150 subunit and belongs to the Ser/Thr protein kinase family. It is a regulatory subunit of the PI3K complex and may regulate membrane trafficking late in the endocytic pathway. This protein can be probably autophosphorylated(PMID:19369195).