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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS18 Monoclonal antibody

VPS18 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E10G11

Cat no : 67590-1-Ig

Synonyms

hVPS18, KIAA1475, PEP3, VPS18

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS18 Monoclonal antibody (67590-1-Ig)

    WB analysis using 67590-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67590-1-Ig (VPS18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VPS18 Monoclonal antibody (67590-1-Ig)

    IHC staining of human liver cancer using 67590-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67590-1-Ig (VPS18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VPS18 Monoclonal antibody (67590-1-Ig)

    IHC staining of human liver cancer using 67590-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67590-1-Ig (VPS18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using VPS18 Monoclonal antibody (67590-1-Ig)

    IF Staining of A549 using 67590-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using VPS18 antibody (67590-1-Ig, Clone: 2E10G11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67590-1-Ig targets VPS18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen VPS18 fusion protein Ag29954 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 18 homolog (S. cerevisiae)
Calculated molecular weight 110 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC001513
Gene symbol VPS18
Gene ID (NCBI) 57617
RRIDAB_2882798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Protocols

Product Specific Protocols
WB protocol for VPS18 antibody 67590-1-IgDownload protocol
IHC protocol for VPS18 antibody 67590-1-IgDownload protocol
IF protocol for VPS18 antibody 67590-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols