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- KD/KO Validated
VPS24 Polyclonal antibody
VPS24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15472-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, human kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
15472-1-AP targets VPS24 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VPS24 fusion protein Ag7776 相同性解析による交差性が予測される生物種 |
| Full Name | vacuolar protein sorting 24 homolog (S. cerevisiae) |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC004419 |
| Gene symbol | VPS24 |
| Gene ID (NCBI) | 51652 |
| RRID | AB_2217102 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS24 antibody 15472-1-AP | Download protocol |
| IHC protocol for VPS24 antibody 15472-1-AP | Download protocol |
| IF protocol for VPS24 antibody 15472-1-AP | Download protocol |
| IP protocol for VPS24 antibody 15472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. | ||
J Cell Biol ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes.
| ||
Int J Oncol CHMP3 promotes the progression of hepatocellular carcinoma by inhibiting caspase‑1‑dependent pyroptosis
| ||
J Extracell Biol RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer |
