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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS28 Recombinant antibody

VPS28 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241886D3

Cat no : 84472-4-RR

Synonyms

Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, 241886D3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS28 Recombinant antibody (84472-4-RR)

    WB analysis using 84472-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84472-4-RR (VPS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VPS28 Recombinant antibody (84472-4-RR)

    IF Staining of HepG2 using 84472-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VPS28 antibody (84472-4-RR, Clone: 241886D3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, THP-1 cells, U-251 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84472-4-RR targets VPS28 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen VPS28 fusion protein Ag7784 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 28 homolog (S. cerevisiae)
Calculated molecular weight 25 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC006485
Gene symbol VPS28
Gene ID (NCBI) 51160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.

Protocols

Product Specific Protocols
WB protocol for VPS28 antibody 84472-4-RRDownload protocol
IF protocol for VPS28 antibody 84472-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols