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  • Featured Product
  • KD/KO Validated

VPS33B Polyclonal antibody, PBS Only

VPS33B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12195-1-PBS

Synonyms

Vacuolar protein sorting-associated protein 33B, hVPS33B

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using VPS33B Polyclonal antibody (12195-1-AP)

    WB analysis of HEK-293 using 12195-1-AP (same clone as 12195-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using VPS33B Polyclonal antibody (12195-1-AP)

    WB analysis of HEK-293 using 12195-1-AP (same clone as 12195-1-PBS)

    WB result of VPS33B antibody (12195-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS33B transfected HEK-293 cells. This data was developed using the same antibody clone with 12195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using VPS33B Polyclonal antibody (12195-1-AP)

    WB analysis of HeLa using 12195-1-AP (same clone as 12195-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using VPS33B Polyclonal antibody (12195-1-AP)

    WB analysis of L02 using 12195-1-AP (same clone as 12195-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using VPS33B Polyclonal antibody (12195-1-AP)

    WB analysis of mouse skeletal muscle using 12195-1-AP (same clone as 12195-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using VPS33B Polyclonal antibody (12195-1-AP)

    WB analysis of mouse testis using 12195-1-AP (same clone as 12195-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VPS33B Polyclonal antibody (12195-1-AP)

    IHC staining of human colon cancer using 12195-1-AP (same clone as 12195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12195-1-AP (VPS33B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VPS33B Polyclonal antibody (12195-1-AP)

    IHC staining of human colon cancer using 12195-1-AP (same clone as 12195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12195-1-AP (VPS33B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VPS33B Polyclonal antibody (12195-1-AP)

    IHC staining of human colon cancer using 12195-1-AP (same clone as 12195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12195-1-AP (VPS33B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12195-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using VPS33B Polyclonal antibody (12195-1-AP)

    IF Staining of MCF-7 using 12195-1-AP (same clone as 12195-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using VPS33B antibody 12195-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12195-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12195-1-PBS targets VPS33B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS33B fusion protein Ag2833 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 33 homolog B (yeast)
Calculated molecular weight 617 aa, 71 kDa
Observed molecular weight 65-71 kDa
GenBank accession numberBC016445
Gene symbol VPS33B
Gene ID (NCBI) 26276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).