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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS36 Polyclonal antibody

VPS36 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14262-1-AP

Synonyms

ELL-associated protein of 45 kDa, EAP45, CGI-145, CGI145, CGI 145

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using VPS36 Polyclonal antibody (14262-1-AP)

    WB analysis of HL-60 using 14262-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14262-1-AP (VPS36 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using VPS36 Polyclonal antibody (14262-1-AP)

    IHC staining of human pancreas cancer using 14262-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14262-1-AP (VPS36 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using VPS36 Polyclonal antibody (14262-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 14262-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14262-1-AP (VPS36 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman pancreas cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14262-1-AP targets VPS36 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS36 fusion protein Ag5549 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 36 homolog (S. cerevisiae)
Calculated molecular weight 44 kDa
Observed molecular weight 43 kDa, 65 kDa
GenBank accession numberBC050439
Gene symbol VPS36
Gene ID (NCBI) 51028
RRIDAB_2878036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VPS36 antibody 14262-1-APDownload protocol
IHC protocol for VPS36 antibody 14262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Neurosci Res

Top2b is involved in the formation of outer segment and synapse during late-stage photoreceptor differentiation by controlling key genes of photoreceptor transcriptional regulatory network.

Authors - Ying Li
View Article
humanWB

Cells

Generation of GLA-Knockout Human Embryonic Stem Cell Lines to Model Autophagic Dysfunction and Exosome Secretion in Fabry Disease-Associated Hypertrophic Cardiomyopathy.

Authors - Hui-Yung Song
View Article