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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS37A Monoclonal antibody, PBS Only

VPS37A Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9A4

Cat no : 66327-1-PBS

Synonyms

ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A

Validation Data Gallery

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  • Western Blot (WB) analysis of SW 1990 cells using VPS37A Monoclonal antibody (66327-1-Ig)

    WB analysis of SW 1990 using 66327-1-Ig (same clone as 66327-1-PBS)

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using VPS37A Monoclonal antibody (66327-1-Ig)

    WB analysis of MCF-7 using 66327-1-Ig (same clone as 66327-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using VPS37A Monoclonal antibody (66327-1-Ig)

    IHC staining of human kidney using 66327-1-Ig (same clone as 66327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using VPS37A Monoclonal antibody (66327-1-Ig)

    IHC staining of human kidney using 66327-1-Ig (same clone as 66327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using VPS37A Monoclonal antibody (66327-1-Ig)

    IHC staining of human ovary tumor using 66327-1-Ig (same clone as 66327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using VPS37A Monoclonal antibody (66327-1-Ig)

    IHC staining of human ovary tumor using 66327-1-Ig (same clone as 66327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using VPS37A Monoclonal antibody (66327-1-Ig)

    IF Staining of MCF-7 using 66327-1-Ig (same clone as 66327-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66327-1-Ig (VPS37A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66327-1-PBS targets VPS37A in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VPS37A fusion protein Ag19108 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 37 homolog A (S. cerevisiae)
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC022363
Gene symbol VPS37A
Gene ID (NCBI) 137492
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.