Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, COLO 320 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
Product Information
15653-1-AP targets VPS37B in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VPS37B fusion protein Ag8256 相同性解析による交差性が予測される生物種 |
| Full Name | vacuolar protein sorting 37 homolog B (S. cerevisiae) |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC005882 |
| Gene Symbol | VPS37B |
| Gene ID (NCBI) | 79720 |
| RRID | AB_2878161 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H9H4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS37B antibody 15653-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy Rep Upregulation of the ESCRT pathway and multivesicular bodies accelerates degradation of proteins associated with neurodegeneration
| ||
EMBO J Mammalian ATG8 proteins maintain autophagosomal membrane integrity through ESCRTs
| ||
J Cell Sci UMAD1 contributes to ESCRT-III dynamic subunit turnover during cytokinetic abscission | ||
Bioorg Chem Chemoproteomics reveals arctigenin as a phagophore-closure blocker via targeting ESCRT-I subunit VPS28 |