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VPS39 Polyclonal antibody
VPS39 Polyclonal Antibody for IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 16219-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human heart tissue, human small intestine tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
IF | See 4 publications below |
IP | See 2 publications below |
Product Information
16219-1-AP targets VPS39 in IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | VPS39 fusion protein Ag9267 相同性解析による交差性が予測される生物種 |
Full Name | vacuolar protein sorting 39 homolog (S. cerevisiae) |
Calculated molecular weight | 886 aa, 102 kDa |
Observed molecular weight | 102 kDa |
GenBank accession number | BC015817 |
Gene symbol | VPS39 |
Gene ID (NCBI) | 23339 |
RRID | AB_11043179 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VPS39, also named as KIAA0770, TLP, or VAM6, is a 886 amino acid protein, which contains one CNH domain and one CHCR repeat. VPS39 belongs to the VAM6/VPS39 family and localizes in the cytoplasm. VPS39 is widely expressed, with highest levels in heart, skeletal muscle, kidney, pancreas, brain, placenta and spleen. VPS39 may play a role in clustering and fusion of late endosomes and lysosomes. VPS39 modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway.
Protocols
Product Specific Protocols | |
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IHC protocol for VPS39 antibody 16219-1-AP | Download protocol |
IP protocol for VPS39 antibody 16219-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy | ||
Cell Host Microbe Global mapping of Salmonella enterica-host protein-protein interactions during infection. | ||
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Dev Cell ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress
| ||
Curr Biol Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy. | ||
Cell Rep Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes |