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VPS41 Polyclonal antibody
VPS41 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 13869-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | RAW 264.7 cells, HepG2 cells, human brain tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| CoIP | See 1 publications below |
Product Information
13869-1-AP targets VPS41 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VPS41 fusion protein Ag4760 相同性解析による交差性が予測される生物種 |
| Full Name | vacuolar protein sorting 41 homolog (S. cerevisiae) |
| Calculated molecular weight | 854 aa, 98 kDa |
| Observed molecular weight | 94-100 kDa |
| GenBank accession number | BC044851 |
| Gene symbol | VPS41 |
| Gene ID (NCBI) | 27072 |
| RRID | AB_2215349 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. VPS41 (vacuolar protein sorting 41) is the human ortholog of yeast Vps41 protein. Yeast Vps41 is a subunit of AP3 (heterotetrameric adaptor protein complex 3) and HOPS (homotypic fusion and protein transport) complexes and is involved in trafficking of proteins from the late Golgi to the vacuole. In mammalian cells, Vps41 was found to be a member of HOPS complex, mediating the trafficking of proteins to lysosomes (PMID: 23173123). It has been reported that human VPS41 has a HOPS-independent function in the LAMP carrier pathway and also participates in lysosomal maturation, probably as part of the HOPS complex (PMID: 23322049).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS41 antibody 13869-1-AP | Download protocol |
| IHC protocol for VPS41 antibody 13869-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy | ||
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Dev Cell ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation.
| ||
J Cell Sci Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L. | ||
J Biol Chem Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity. | ||
J Cell Sci The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome.
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