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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS45 Polyclonal antibody (12006-1-AP)

    IHC staining of mouse brain using 12006-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS45 Polyclonal antibody (12006-1-AP)

    IHC staining of mouse brain using 12006-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using VPS45 Polyclonal antibody (12006-1-AP)

    IHC staining of human testis using 12006-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using VPS45 Polyclonal antibody (12006-1-AP)

    IHC staining of human testis using 12006-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using VPS45 Polyclonal antibody (12006-1-AP)

    IHC staining of human heart using 12006-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using VPS45 Polyclonal antibody (12006-1-AP)

    IHC staining of human heart using 12006-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VPS45 Polyclonal antibody (12006-1-AP)

    IF Staining of HeLa using 12006-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12006-1-AP (VPS45 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VPS45 Polyclonal antibody (12006-1-AP)

    IF Staining of HepG2 using 12006-1-AP

    Immunofluorescent analysis of HepG2 cells, using VPS45 antibody 12006-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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VPS45 Polyclonal antibody

VPS45 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 12006-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

Vacuolar protein sorting-associated protein 45, h-VPS45, hlVps45, H1VPS45, H1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse brain tissue, human testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12006-1-AP targets VPS45 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS45 fusion protein Ag2658 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 45 homolog (S. cerevisiae)
Calculated molecular weight 570 aa, 65 kDa
GenBank accession numberBC012932
Gene Symbol VPS45
Gene ID (NCBI) 11311
RRIDAB_2215369
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRW7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)

Protocols

Product Specific Protocols
IHC protocol for VPS45 antibody 12006-1-APDownload protocol
IF protocol for VPS45 antibody 12006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Gene

Rice protein suppresses ROS generation and stimulates antioxidant gene expression via Nrf2 activation in adult rats.

Authors - Hui Li
View Article