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  • Featured Product
  • KD/KO Validated

VPS4A Polyclonal antibody

VPS4A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14272-1-AP

Synonyms

hVPS4, Protein SKD2, SKD1, SKD2, VPS4, VPS4 1, VPS4A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS4A Polyclonal antibody (14272-1-AP)

    WB analysis using 14272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VPS4A Polyclonal antibody (14272-1-AP)

    WB analysis using 14272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using VPS4A Polyclonal antibody (14272-1-AP)

    WB analysis of U-937 using 14272-1-AP

    WB result of VPS4A antibody (14272-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS4A transfected U-937 cells.

  • Western Blot (WB) analysis of HepG2 cells using VPS4A Polyclonal antibody (14272-1-AP)

    WB analysis of HepG2 using 14272-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using VPS4A Polyclonal antibody (14272-1-AP)

    WB analysis of SMMC-7721 using 14272-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using VPS4A Polyclonal antibody (14272-1-AP)

    WB analysis of SMMC-7721 using 14272-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using VPS4A Polyclonal antibody (14272-1-AP)

    IHC staining of human kidney using 14272-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using VPS4A Polyclonal antibody (14272-1-AP)

    IHC staining of human kidney using 14272-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inU-937 cells, SMMC-7721 cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14272-1-AP targets VPS4A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS4A fusion protein Ag5592 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 4 homolog A (S. cerevisiae)
Calculated molecular weight 49 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberBC047932
Gene symbol VPS4A
Gene ID (NCBI) 27183
RRIDAB_2878039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VPS4A antibody 14272-1-APDownload protocol
IHC protocol for VPS4A antibody 14272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineWB

Parasitol Res

Microautophagy upregulation in cutaneous lymph nodes of dogs naturally infected by Leishmania infantum.

Authors - Francesca De Falco
View Article
humanWB

Theranostics

Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy

Authors - Kunjian Lei
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Nuo (Verified Customer) (11-08-2024)

This antibody can detect endogenous VPS4A with a MW of around 50 kD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse brain lysate