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  • Featured Product
  • KD/KO Validated

VPS4A/B Polyclonal antibody, PBS Only

VPS4A/B Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17673-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS4A/B Polyclonal antibody (17673-1-AP)

    WB analysis using 17673-1-AP (same clone as 17673-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skin tissue using VPS4A/B Polyclonal antibody (17673-1-AP)

    WB analysis of mouse skin using 17673-1-AP (same clone as 17673-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using VPS4A/B Polyclonal antibody (17673-1-AP)

    IP experiment of HeLa using 17673-1-AP (same clone as 17673-1-PBS)

    IP result of anti-VPS4A/B (IP:17673-1-AP, 4ug; Detection:17673-1-AP 1:3000) with HeLa cells lysate 1360 ug. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using VPS4A/B Polyclonal antibody (17673-1-AP)

    IF Staining of A431 using 17673-1-AP (same clone as 17673-1-PBS)

    Immunofluorescent analysis of A431 cells using 17673-1-AP (VPS4B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17673-1-PBS targets VPS4A/B in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS4A/B fusion protein Ag11610 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 4 homolog B (S. cerevisiae)
Calculated molecular weight 444 aa, 49 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC039574
Gene symbol VPS4B
Gene ID (NCBI) 9525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. Human VPS4 has two orthologous proteins named VPS4A and VPS4B. This antibody can recognize both VPS4A and VPS4B due to their high homology.