Validation Data Gallery

Filter: AllWBIPFC (Intra)

WB analysis using 17673-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skin using 17673-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17673-1-AP
IP result of anti-VPS4A/B (IP:17673-1-AP, 4ug; Detection:17673-1-AP 1:3000) with HeLa cells lysate 1360 ug.
FC experiment of A431 using 17673-1-AP
1x10^6 A431 cells were intracellularly stained with 0.25 ug VPS4A/B Polyclonal antibody (17673-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

VPS4A/B Polyclonal antibody

VPS4A/B Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Cat No. 17673-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC (Intra), IP, ELISA

Synonyms

VPS4B, Vacuolar protein sorting-associated protein 4B, SKD1, Protein SKD1, MIG1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (11)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, mouse skin tissue, HEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IP detected in	HeLa cells
Positive FC (Intra) detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 9 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

17673-1-AP targets VPS4A/B in WB, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VPS4A/B fusion protein Ag11610 相同性解析による交差性が予測される生物種
Full Name	vacuolar protein sorting 4 homolog B (S. cerevisiae)
Calculated molecular weight	444 aa, 49 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC039574
Gene Symbol	VPS4B
Gene ID (NCBI)	9525
RRID	AB_2215504
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75351
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. Human VPS4 has two orthologous proteins named VPS4A and VPS4B. This antibody can recognize both VPS4A and VPS4B due to their high homology.

Protocols

Product Specific Protocols
WB protocol for VPS4A/B antibody 17673-1-AP	Download protocol
IP protocol for VPS4A/B antibody 17673-1-AP	Download protocol
FC protocol for VPS4A/B antibody 17673-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res RAB31 marks and controls an ESCRT-independent exosome pathway. Authors - Denghui Wei View Article
human	IF	Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. Authors - Vincent Mercier View Article
rat	WB,IF	EMBO J TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons. Authors - Benjamin M Schwenk View Article
human	WB	J Cell Sci His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation Authors - Gabrielle Parkinson View Article
human	WB	Viruses Characterization of Bovine Foamy Virus Gag Late Assembly Domain Motifs and Their Role in Recruiting ESCRT for Budding. Authors - Zhaohuan Wang View Article
human	WB,IF	Nucleus VPS4B orchestrates response to nuclear envelope stress by regulating ESCRT-III dynamics in glioblastoma Authors - Zuqian Wu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17673-1-AP

WB analysis of mouse skin using 17673-1-AP

IP experiment of HeLa using 17673-1-AP

FC experiment of A431 using 17673-1-AP

VPS4A/B Polyclonal antibody

VPS4A/B Polyclonal Antibody for WB, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

RAB31 marks and controls an ESCRT-independent exosome pathway.

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons.

His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation

Characterization of Bovine Foamy Virus Gag Late Assembly Domain Motifs and Their Role in Recruiting ESCRT for Budding.

VPS4B orchestrates response to nuclear envelope stress by regulating ESCRT-III dynamics in glioblastoma